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. 2009 Oct 28;4(10):e7650. doi: 10.1371/journal.pone.0007650

Figure 6. BMP signaling is not required for cardiogenesis in AC/AE model.

Figure 6

(A) Animal caps expressing truncated type I BMP receptor (tBR) or Cerberus (1 ng of mRNA each), were conjugated with anterior endoderm and conjugates were analyzed for expression of MHCα and N-tubulin at st. 34 by RT-PCR. (B) Quantification of RT-PCR in A. Both anti-BMP reagents used were active, as shown by induction of N-tubulin expression, but had no effect on cardiogenesis. (C) Dose-dependent inhibition of cardiogenesis in AC/AE explants by Cerberus. Animal caps from embryos injected with 1, 2 or 4 ng of Cerberus mRNA were conjugated with anterior endoderm and conjugates were analyzed for MHCα expression when sibling control embryos reached st. 34. The effect of Cerberus was quantified and normalized to control conjugates (% C AC/AE). (D) Phenotypic controls for Cerberus mRNA, showing dose-dependent increase in cement gland tissue.