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. 2009 Oct 26;4(10):e7592. doi: 10.1371/journal.pone.0007592

Figure 5. RNA expression of NMDA, AMPA, KA and GABAA receptors in Tg20, Prnp −/− and wild-type mice.

Figure 5

A) Agarose gel showing the amplified products of mice genotyping. B) Histograms showing the level of expression of Prnp mRNA obtained by RT-qPCR in each genotype (Tg20, Prnp −/− and wild-type). C) Agarose gel of the amplified products using the Sybr Green RT-qPCR primers for GluR1, GluR2, GluR6, GluR7, KAR1, KAR2, NR1, NR2A, NR2B, GABAA-α1, GABAA-δ, GABAA-γ2 and GAPDH. The size of the amplified bands is indicated in each lane in B. GADPH was used as internal control. D) Examples of the melting point analysis of the amplified products for GluR6, GluR7, NR2A, GABAA-γ2 and GADPH. Note the presence of different curves for each gene. The mean temperature for GABAA-γ2 is shown as an example. E) Histogram illustrating the quantitative results of the RT-qPCR experiment of target mRNA levels. Histograms represent the mean ± SEM of three independent experiments correlating receptor subunits/GADPH levels by using the 2−ΔΔCt method. Fold increases or decreases were calculated with respect to wild-type data.