FIG. 6.

Effects of oxidative stress on Cx56 in cultured embryonic chicken lens cells. (A) Immunoblot of Cx56 from homogenates of lentoid-containing cultures that were left untreated (Control) or that were treated with 100 or 500 μM H₂O₂ for 3 h. (B) Immunoblot of Cx56 from homogenates of lentoid-containing cultures that were left untreated (Control) or that were treated with 500 μM H₂O₂ for 30 min or 3 h. The migration positions of the molecular mass standards are indicated. Only subtle changes in the immunoblot pattern of Cx56 bands were detected after treatment with 100 μM H₂O₂ for 3 h. A prominent degradation product of Cx56 was generated after treatment with 500 μM H₂O₂ for 3 h.