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. Author manuscript; available in PMC: 2010 Mar 17.
Published in final edited form as: Oncogene. 2009 Jul 13;28(37):3286–3295. doi: 10.1038/onc.2009.187

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The therapeutic gene BikDD was expressed in lung cancer cells and inhibited lung cancer cells growth. (A) The schematic diagram of therapeutic constructs. (B) BikDD driven by CMV and SV was detected in F4 lung cancer cell lysate after 24h plasmids transient transfection by Western blot. (C) In vitro cell killing effect of BikDD. Indicated therapeutic plasmids and pRL-TK were co-transfected into cells by lipofetamine 2000. The renilla luciferase activity was detected after 48 hours. Relative cell viability was measured by setting Ctrl as 100% cell viability. The p values between lung cancer cell lines and normal cell lines with CMV-BikDD and SV-BikDD treatment were 0.19 and 0.006, respectively.