Table 2.

Point-to-plane depth restraints used for membrane docking.

mutant	Φ	distance (Å)	Range (Å)
L171R1	−1.9	−4.0	−43.0 < x < 0.0
M173R1	+0.8	+7.8	+6.9 < x < +8.6
G174R1	+0.5	+6.9	+6.7 < x < +7.2
†K189R1	−1.8	−3.0	−43.0 < x < −2.1
L202R1	−2.5	−22.0	−43.0 < x < −5.0
F231R1	−1.4	0.0	−4.0 < x < +2.4
F234R1	−0.1	+5.2	+4.9 < x < +5.5
K236R1	−0.3	+4.6	+3.9 < x < +5.2
I239R1	−1.5	−0.6	−3.0 < x < +1.1
†V283R1	−1.9	−4.0	−43.0 < x < −3.0
†T285R1	−1.4	0.0	−4.0 < x < +2.4
K300R1	−1.7	−2.1	−5.4 < x < 0
V304R1	−0.4	+4.3	+3.6 < x < +4.9
G305R1	+0.8	+7.8	+7.2 < x < +8.3
G306R1	+0.3	+6.4	+5.5 < x < +7.2
K327R1	−2.2	−10.6	−43.0 < x < −5.4
T334R1	−2.2	−10.6	−43.0 < x < −7.3
I367R1	−2.1	−7.3	−43.0 < x < −2.1
G368R1	+0.6	+7.2	+6.9 < x < +7.5
K369R1	−1.2	+1.1	0.0 < x < +2.0
N370R1	−2.3	−21.1	−43.0 < x < −5.4

Distances were determined from measured values of Φ as described elsewhere ⁹; ⁵⁰. The distances represent the distance from the nitrogen atom on the nitroxide R1 side chain to the plane that defines the average position of the lipid phosphates. Negative distance numbers correspond to labels on the aqueous side of the phosphate plane, positive numbers represent labels that lie on the hydrocarbon side. Most of the point-to-plane distances given in this table were reported previously and were determined using Ni(II)EDDA and oxygen collision parameter ¹⁰. Values for three sites were determined here (†). The distance ranges are based upon the error in Φ and the form of the calibration curve (see Methods).