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. 1966 Oct;92(4):851–855. doi: 10.1128/jb.92.4.851-855.1966

Influence of Phosphate Starvation on Cultures of Pseudomonas aeruginosa

Cynthia I Hou 1, Audrey F Gronlund 1, J J R Campbell 1
PMCID: PMC276344  PMID: 4959048

Abstract

Hou, Cynthia I. (University of British Columbia, Vancouver, B.C., Canada), Audrey F. Gronlund, and J. J. R. Campbell. Influence of phosphate starvation on cultures of Pseudomonas aeruginosa. J. Bacteriol. 92:851–855. 1966.—The changes occurring in Pseudomonas aeruginosa during phosphate starvation in a phosphate-deficient medium were assessed by measuring alterations in optical density, viable-cell count, chemical composition, and ribosome patterns. After a 24-hr period of starvation, optical density, protein, and deoxyribonucleic acid per milliliter of culture increased, whereas ribonucleic acid decreased. Extensive ribosomal degradation was apparent from sucrose density gradient centrifugation patterns. The induction of an alkaline phosphomonoesterase during phosphate starvation was observed. A linear response of phosphate-starved cells to low levels of phosphate supplied exogenously was evident from optical-density measurements, and a threshold requirement for phosphate analogous to the “energy of maintenance” was not detected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CAMPBELL J. J., GRONLUND A. F., DUNCAN M. G. Endogenous metabolism of Pseudomonas. Ann N Y Acad Sci. 1963 Jan 21;102:669–677. doi: 10.1111/j.1749-6632.1963.tb13667.x. [DOI] [PubMed] [Google Scholar]
  2. GAREN A., LEVINTHAL C. A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase. Biochim Biophys Acta. 1960 Mar 11;38:470–483. doi: 10.1016/0006-3002(60)91282-8. [DOI] [PubMed] [Google Scholar]
  3. GRONLUND A. F., CAMPBELL J. J. NITROGENOUS SUBSTRATES OF ENDOGENOUS RESPIRATION IN PSEUDOMONAS AERUGINOSA. J Bacteriol. 1963 Jul;86:58–66. doi: 10.1128/jb.86.1.58-66.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gronlund A. F., Campbell J. J. Enzymatic Degradation of Ribosomes During Endogenous Respiration of Pseudomonas aeruginosa. J Bacteriol. 1965 Jul;90(1):1–7. doi: 10.1128/jb.90.1.1-7.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HEPPEL L. A., HARKNESS D. R., HILMOE R. J. A study of the substrate specificity and other properties of the alkaline phosphatase of Escherichia coli. J Biol Chem. 1962 Mar;237:841–846. [PubMed] [Google Scholar]
  6. HUTCHISON W. C., DOWNIE E. D., MUNRO H. N. Factors affecting the Schneider procedure for estimation of nucleic acids. Biochim Biophys Acta. 1962 May 14;55:561–570. doi: 10.1016/0006-3002(62)90835-1. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. MALLETTE M. F., COWAN C. I., CAMPBELL J. J. GROWTH AND SURVIVAL OF ESCHERICHIA COLI IN MEDIUM LIMITED IN PHOSPHATE. J Bacteriol. 1964 Apr;87:779–785. doi: 10.1128/jb.87.4.779-785.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McGrew S. B., Mallette M. F. ENERGY OF MAINTENANCE IN ESCHERICHIA COLI. J Bacteriol. 1962 Apr;83(4):844–850. doi: 10.1128/jb.83.4.844-850.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. TORRIANI A. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim Biophys Acta. 1960 Mar 11;38:460–469. doi: 10.1016/0006-3002(60)91281-6. [DOI] [PubMed] [Google Scholar]

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