Table 1.
M. extorquens AM1 strains and plasmids used in this study
| Strain | Description | Source or reference |
|---|---|---|
| AM1 | RifR derivative | Lab collection |
| CM174 | katA : : (loxP-trrnB-PmxaF-gfpuv-tT7) | Marx & Lidstrom (2004) |
| TSG.10 | katA : : (loxP-trrnB-Plac-gfpuv-tT7) | This study |
| TSG.11-2 | katA : : (loxP-trrnB-PphaA-gfpuv-tT7) | This study |
| TSG.12-2 | katA : : (loxP-trrnB-PsgaA-gfpuv-tT7) | This study |
| TSX | Non-motile mutant (ΔmotA-fliN-fliM-fliG, ΔmotC-motB, ΔpomA-motB) | Strovas et al. (2007) |
| TSXCM174 | Non-motile mutant with katA : : (loxP-trrnB-PmxaF-gfpuv-t17) | Strovas et al. (2007) |
| Plasmid | ||
| pGFPuv | Commercial vector | BD Biosciences (ClonTech) |
| pCR2.1 | PCR cloning vector | Invitrogen |
| pCM139 | Broad-host-range shuttle vector containing Pfae-fae | Vorholt et al. (2000) |
| pCM157 | Broad-host-range cre expression vector (TetR) | Marx & Lidstrom (2002) |
| pCM168 | Insertional expression vector (ttrrnB-MCS-tT7) | Marx & Lidstrom (2004) |
| pCM174 | pCM168 with PmxaF-gfpuv | Marx & Lidstrom (2004) |
| pRK2073 | Helper plasmid expressing IncP tra functions | Lab collection |
| pTS29 | pCR2.1 with PphaA | This study |
| pTS35r | pCR2.1 with PsgaA | This study |
| pTSGex | pCM139 with Plac-gfpuv | This study |
| pTSG.10 | pCM168 with Plac-gfpuv | This study |
| pTSG.11-2 | pCM168 with PphaA-gfpuv | This study |
| pTSG.12-2 | pCM168 with PsgaA-gfpuv | This study |