Abstract
Rudbach, Jon A. (The University of Michigan, Ann Arbor), and Arthur G. Johnson. Alteration of endotoxin activity after complexing with plasma proteins. J. Bacteriol. 92:892–898. 1966.—A substantial decrease in the ability of endotoxin to be precipitated by homologous antiserum and to cause fever occurred after incubation with human plasma or human plasma, Cohn fraction IV-1. The endotoxin, thus altered, also displayed decreased lethality for rabbits. These alterations in endotoxin activity could be restored when the endotoxin-plasma protein mixture was treated with a proteolytic enzyme, and the endotoxin was precipitated with ethyl alcohol. Inactivation of the antigenic and toxigenic properties of the endotoxin molecule by plasma is discussed as resulting from complexing with plasma proteins rather than from enzymatic degradation.
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- ATKINS E. Pathogenesis of fever. Physiol Rev. 1960 Jul;40:580–646. doi: 10.1152/physrev.1960.40.3.580. [DOI] [PubMed] [Google Scholar]
- BRAUDE A. I., CAREY F. J., SUTHERLAND D., ZALESKY M. Studies with radioactive endotoxin. I. The use of Cr51 to label endotoxin of Escherichia coli. J Clin Invest. 1955 Jun;34(6):850–857. doi: 10.1172/JCI103140. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CLUFF L. E., BENNETT I. L., Jr Factors influencing the alteration of the pyrogenic action of endotoxin by serum. Bull Johns Hopkins Hosp. 1957 Nov;101(5):281–291. [PubMed] [Google Scholar]
- FARR R. S., LeQUIRE V. S. Leukocytic and pyrogenic effects of typhoid vaccine and augmentation by homologous plasma. Proc Soc Exp Biol Med. 1950 Dec;75(3):661–666. doi: 10.3181/00379727-75-18298. [DOI] [PubMed] [Google Scholar]
- HEGEMANN F. Zur Bedeutung des Blutserums für die Entstehung und das Unwirksamwerden bakterieller Reizstoffe beim Menschen. II. Die neutralisierende Wirkung des menschlichen Serums auf das Endotoxin von Colibakterien. Z Immun exp ther. 1954 Aug;111(3):213–225. [PubMed] [Google Scholar]
- LANDY M. Inactivation of endotoxin by humoral systems. Ann N Y Acad Sci. 1960 Nov 21;88:1273–1277. doi: 10.1111/j.1749-6632.1960.tb20118.x. [DOI] [PubMed] [Google Scholar]
- LANDY M., TRAPANI R. J., SHEAR M. J. Inactivation of endotoxin by a humoral component. IV. Alteration in the immunological properties of typhoid endotoxin. J Exp Med. 1959 Nov 1;110:731–750. doi: 10.1084/jem.110.5.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OROSZLAN S. I., MORA P. T. DISSOCIATION AND RECONSTITUTION OF AN ENDOTOXIN. Biochem Biophys Res Commun. 1963 Aug 14;12:345–349. doi: 10.1016/0006-291x(63)90102-5. [DOI] [PubMed] [Google Scholar]
- OROSZLAN S. I., MORA P. T., SHEAR M. J. REVERSIBLE INACTIVATION OF AN ENDOTOXIN BY INTRACELLULAR PROTEIN. Biochem Pharmacol. 1963 Oct;12:1131–1146. doi: 10.1016/0006-2952(63)90087-x. [DOI] [PubMed] [Google Scholar]
- RUDBACH J. A., JOHNSON A. G. Changes in serologic reactivity of endotoxin induced by fraction IV-1 (Cohn) of normal human serum. Proc Soc Exp Biol Med. 1962 Dec;111:651–655. doi: 10.3181/00379727-111-27882. [DOI] [PubMed] [Google Scholar]
- RUDBACH J. A., JOHNSON A. G. RESTORATION OF ENDOTOXIN ACTIVITY FOLLOWING ALTERATION BY PLASMA. Nature. 1964 May 23;202:811–812. doi: 10.1038/202811a0. [DOI] [PubMed] [Google Scholar]
- STAUCH J. E., JOHNSON A. G. The alteration of bacterial endotoxins by human and rabbit serum. J Immunol. 1959 Mar;82(3):252–263. [PubMed] [Google Scholar]
- VANVUNAKIS H., RUFFILLI A., LEVINE L. ENHANCEMENTS OF ANTIBIOTIC ACTIVITY OF COLICINE K AND ALTERATION OF SEROLOGICAL PROPERTIES OF COLICINE K AND ENDOTOXINS. J Exp Med. 1965 Feb 1;121:261–278. doi: 10.1084/jem.121.2.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YOSHIOKA M., HAMASAKA M., SAITO Y., KONNO S. CHARACTERISTICS OF ENDOTOXIN ALTERING FRACTIONS DERIVED FROM NORMAL SERUM. I. SEPARATION OF BETA LIPOPROTEINS AND LIPID FROM HUMAN SERUM FRACTIONS. Kitasato Arch Exp Med. 1963 Dec;36:27–36. [PubMed] [Google Scholar]
- YOSHIOKA M., JOHNSON A. G. Characteristics of endotoxin altering fractions derived from normal human serum. J Immunol. 1962 Sep;89:326–335. [PubMed] [Google Scholar]