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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Evol Dev. 2009 Jul-Aug;11(4):376–381. doi: 10.1111/j.1525-142X.2009.00344.x

Table 1.

Primer sequences used for bindin amplification

Name 5’-Sequence-3’ Codons Application1 Target
AGCf GACACTACATGTCCTGCAGGATGTCG 25-33 RACE, cDNA preprobindin
AGCf2 CATGTCCTGCAGGATGTCG 27-33 gDNA preprobindin
TALf GGTCATTGTATCTACCAACAGCGCTGG 188-197 RACE preprobindin, 1st repeat cluster
AAEf GGCACGACGTGCCGCAGAG 227-233 RACE, gDNA 1st repeat
AAEr CTCTGCGGCACGTCGTGCC 233-227 gDNA preprobindin
VEGKf GTTAAACCAGTGGAAGGGAAGC 407-414 gDNA 2nd repeat cluster
VEGKr GCTTCCCTTCCACTGGTTTAAC 414-407 gDNA 1st repeat
QPAf GGAATCGGAGTCACAACCAGCGG 925-933 RACE, gDNA ‘hot spot’, intron
QPAr CCGCTGGTTGTGACTCCGATTCC 933-925 gDNA non-repetitive sequence, 2nd repeat
DLPf GGAATCTACGAAGATTGACCTTCCTGG 967-976 RACE core region, 3’ UTR
VLSr CGTCGTTTTCATTCATGGCGCTGAGG 1019-1010 RACE, cDNA core region
VLSr2 GTTTTCATTCATGGCGCTGAGG 1017-1010 gDNA core region, intron
1

Primers were used for rapid amplification of cDNA ends (RACE), or in forward (f) and reverse (r) pairs for targeted amplification of the coding sequence only (cDNA) or the genomic sequence (gDNA).