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Effect of capillary coating on run reproducibility. Conditions: BGE, 400 mM PFPA adjusted to pH 2 with LiOH. Coating procedure: prerun wash with INSTCoating × 20 psi (137.9 kPa) × 5 min followed by BGE × 80 psi (551.6 kPa) × 5 min. After each run the capillary is washed with water × 80 psi (551.6 kPa) × 5 min. The peptide sequence is Ac-XLGLGGGLGLGK-OH, where position X is substituted by amino acids indicated by their one-letter code.
