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. Author manuscript; available in PMC: 2009 Oct 19.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Feb 8;368(3):801–807. doi: 10.1016/j.bbrc.2008.02.001

Fig. 2. CKIε inhibition prevents phosphorylation of the Akt target GSK3β.

Fig. 2

MCF7 cells were transiently transfected with CKIε and/or incubated with insulin as shown below. Pathway activation was measure by phosphorylation of GSK3β at Ser 9 (bottom 2 bands). Inhibition of CKIε by IC261 resulted in GSK3β phosphorylation levels similar to those in the absence of insulin, suggesting that both may be required for maximum signal strength.