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. Author manuscript; available in PMC: 2009 Oct 19.
Published in final edited form as: Traffic. 2008 Aug 9;9(11):1972–1983. doi: 10.1111/j.1600-0854.2008.00817.x

Figure 2. Ubiquitin fusion to the C-terminus of EIAV Gag lacking a late domain rescues virus particle production.

Figure 2

293T cells were transfected with plasmids expressing the EIAV Gag proteins depicted in Figure 1 and were labeled with [35S]Met/Cys for 5 h. Cell and VLP lysates were immunoprecipitated with horse anti-EIAV serum followed by resolution on SDS-PAGE gels. Levels of cell- and virion-associated Gag proteins were quantified by phosphorImager analysis. VLP release efficiency was calculated as (VLP Gag)/[total (cell + VLP) Gag] as a percentage of EIAV/WT release. Data represent mean ± SD, n = 6.