Table 2.

Substrate Specificity of FANCJ Helicase

Substrate Name	Substrate Structure	Relative Helicase Activity
3'-tailed duplex a		No
5'-tailed duplex a		+
Forked duplex a		+++
5' flap substrate a		+++
3' flap substrate a		No
Replication fork a		No
Holliday junction a		No
D-loop with a 5' ssDNA tail a		+++
D-loop with a 3' ssDNA tail a		++
Flush D-loop a		+
G-quadruplex DNA b		++++
M13 partial duplex DNA c		++
M13 partial DNA-RNA hybrid c		+
RNA double strand c		No

^aSee text and ref 63

^bref 76

^cref 62 for details.