Comparison of selectivity of control MB and modified MBs. A final concentration of 50 nM MB was incubated with 500 nM of each target for 0.5 h and the fluorescence signal was measured. The experiment was repeated three times and the average value was calculated. (a) MBs (lane 1, normalized as zero); MBs incubate with the targets (6mer, 8mer, 10mer, and 12mer target, respectively, lane 2, 3, 4, and 5); MBs incubate with the loop targets (lane 6, normalized as one). (b) MBs (lane 1, normalized as zero); MBs incubate with the targets (ZP-10mer and ZP-12mer target, lane 2 and 3); MBs incubate with the loop targets (lane 4, normalized as one).