Table 1.
Sequences of MBs and targets used in this study
| Name | Sequence |
|---|---|
| Control MB | 5'-FAM-CCTAGCTCTAAATCACTATGGTCGCGCTAGG-Dabcyl-3' |
| ZPMB-1 | 5'-FAM-CCTAGZTCTAAATCACTATGGTCGCPCTAGG-Dabcyl-3' |
| ZPMB-2Ca | 5'-FAM-CCTAPZTCTAAATCACTATGGTCGCPZTAGG-Dabcyl-3' |
| ZPMB-2Sb | 5'-FAM-CZTAPCTCTAAATCACTATGGTCGCGZTAPG-Dabcyl-3' |
| ZPMB-3 | 5'-FAM-CZTAPZTCTAAATCACTATGGTCGCPZTAPG-Dabcyl-3' |
| Loop Target | 3'-AGATTTAGTGATACCAGCG-5' |
| 6mer Target | 3'-CGATCC-5' |
| 8mer Target | 3'-CGCGATCC-5' |
| 10mer Target | 3'-AGCGCGATCC-5' |
| 12mer Target | 3'-CCAGCGCGATCC-5' |
| ZP-10mer Target | 3'-GPATZGAGAT-5' |
| ZP-12mer Target | 3'-GPATZGAGATTT-5' |
a
2C Indicates that the MB has two consecutively modified nucleobases in the stem.
b
2S Indicates that the MB has two separately modified nucleobases in the stem. Underlined regions are stems and their targets. The presence of Z in the synthetic oligonucleotides was confirmed by UV absorbance at 390 nm.