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. Author manuscript; available in PMC: 2009 Oct 19.
Published in final edited form as: Nat Protoc. 2008;3(4):719–738. doi: 10.1038/nprot.2008.40

Figure 6.

Figure 6

Seeding methods. (a) Cell inoculation using Matrigel. (b) H&E-stained cross-section of a poly(glycerol sebacate) (PGS) scaffold immediately following neonatal rat heart cell inoculation with Matrigel. (c) Set up for perfusion seeding using alternating culture medium flow. (1) Gas exchanger, (2,4) de-bubbling syringes, (3) perfusion chamber, (5) Syringe pump. (d) H&E-stained cross-section of a collagen scaffold inoculated with 12 million C2C12 cells using Matrigel and seeded under alternating flow perfusion at 1.5 ml min−1 for 4.5 h. (e) Perfusion loop for cell seeding consisting of a multichannel peristaltic pump, (1) filters, (2, 3) U shaped tubing, (4) injection sites, (5) three-way stopcocks, (6) Apollo perfusion chambers with tissue construct. In the perfusion chamber, the construct was squeezed between (7) two silicone gaskets to force the flow through the 5 mm diameter scaffold core. (f) H&E-stained cross-section of a construct after 2 h of perfusion seeding of cardiac cells.