Figure 7.

Tissue culture in a generalized perfusion loop. (a) Open perfusion chamber. (b) Insert a Matrigel-inoculated scaffold into a primed perfusion cartridge. (c) Insert a silicone gasket on top of the inoculated scaffold. (d) Insert the stainless steel screen into the perfusion chamber. (e) De-bubble by placing the perfusion chamber in vertical position such that a gas bubble is at a higher point than the scaffold. Apply the culture medium from the de-bubbling syringe below the scaffold. (f) Primed bubble-free perfusion chamber with cell-seeded scaffold in place (1) stainless steel screen, (2) cell-seeded scaffolds, and (3) silicone gasket. (g) Place the perfusion loop into the incubator. Note that these pictures were taken on a bench for better clarity; however, this procedure should be performed in a biosafety cabinet over a sterile Petri dish.