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. Author manuscript; available in PMC: 2009 Oct 19.
Published in final edited form as: J Endod. 2008 Sep 26;34(12):1470–1473. doi: 10.1016/j.joen.2008.08.027

Figure 1.

Figure 1

Pedigree and mutational analyses of the kindred. (A) Pedigree of the kindred. Black arrow indicates proband. A + identifies members recruited in this study. (B) DNA sequencing chromatogram of the affected individual III-4. Red arrow indicates the position of the mutation. (C) DNA sequencing chromatogram of the unaffected individual III-3. (D) Agarose gel image of in vitro splicing assay. Left lane is DNA size marker. Middle lane is a result of pSPL3 vector with normal insert. Right lane is a result of pSPL3 vector with mutated insert. Upper band in the right lane is normally spliced mRNA transcript, and lower band is abnormally spliced mRNA with exon 3 deletion.