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. 2009 Aug 5;29(31):9704–9713. doi: 10.1523/JNEUROSCI.2292-09.2009

Figure 3.

Figure 3.

Alterations of synaptic proteins from extracellular Aβ1-42 occur via γ-secretase processing of APP. a, Treatment of wild-type neurons with Aβ1-42 increased levels of intraneuronal Aβ42 and reduced PSD-95 puncta as evident by immunofluorescence (central panels), but cotreatment with DAPT prevented these effects of Aβ1-42 (right panels). b, Quantitation of Aβ42 staining expressed as percentage of the Aβ42 immunofluorescence in the control (n = 5). c, Quantitation of PSD-95 expressed as a percentage of PSD-95 immunofluorescence of the control (n = 5). d, Treatment of wild-type neurons with Aβ1-42 reduced levels of surface NR1 as measured by surface biotinylation followed by Western blot. e, Quantitation of the ratio of surface NR1 to total NR1 in Aβ1-42 treated compared with untreated neurons and Aβ1-42 + DAPT treated compared with untreated neurons (n = 4). f, Treatment of APP KO neurons with Aβ1-42 or Aβ1-42 + DAPT showed unchanged levels of PSD-95 puncta as evident by immunofluorescence. **p < 0.01; scale bar: 10 μm.