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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: FEMS Microbiol Lett. 2009 Feb 26;293(2):278–284. doi: 10.1111/j.1574-6968.2009.01542.x

Figure 3. Effect of deletions and mutations of the flaB promoter on gene expression.

Figure 3

Panels A and C. To ensure that equivalent amounts of total protein from each sample were assayed, the same volume of lysate from each sample was electrophoresed through an SDS-polyacrylamide and stained with Coomassie Blue R250 dye. Panels B and D. Western blots showing the relative expression of GFP from the different promoter-gfp constructs. The expression of GFP was detected using a rabbit anti-GFP antibody. Two transformants carrying the following constructs, pBSV2G-flaBp2-gfp (lanes 1 and 2), pBSV2G-flaBp35-gfp (lanes 3 and 4) and pBSV2G-flaBp3-gfp (lanes 5 and 6), were tested (Panel B). Similarly, GFP expression was assayed from transformants carrying the following mutations within the flaB promoter: pBSV2G-flaBp2-gfp (lanes 1 and 2) and pBSV2G-flaBp2sm-gfp (lanes 3 and 4), and pBSV2G-flaBp2C→G-gfp (lanes 5 and 6). Lane C is a promoterless negative control (pBSV2G-gfp) (Panel D).