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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: FEMS Microbiol Lett. 2009 Feb 26;293(2):278–284. doi: 10.1111/j.1574-6968.2009.01542.x

Table 1. Primers used in this study.

Primer Sequencea Gene(s)/Plasmid Use
T116 TAATTATTTTAATGCTATTGCTATTTGCGTTTCTTTTTTTTTAA flaBp pBSV2G-flaBp2-gfp
T123 TTTTTTTTAATTTTTGTGCTATTCTTTTTAACAGGCAAAAGGATT flaBp pBSV2G-flaBp3-gfp
T230 TTAATGCTATTGCTATTTGCGTTTCTTTTTTTTTAATTTTTGTGCCATTCTTTTTAAC flaBp pBSV2G-flaBp2T→C-gfp
T240 gcatgcaccggtaccTAATTATTTTTAATGCTATTGCTATTTGCG flaBp Multiple constructs
T316 tcccccgggATGCTATTGCTATTTGCGTTTCTTTTTGTTGGATTGTTGTGCTATTCT flaBp pBSV2G-flaBp2sm-gfp
T317 tcccccgggATGCTATTGCTATTTGCGTTTCTTTTTTTTTAATTTTTGTGGTATTCT flaBp pBSV2G-flaBp2C→G-gfp
T319 gatcagataggtaccCTTCTTTTTTTTTAATTTTTGTGC flaBp pBSV2G-flaBp35-gfp
B93 cgatagcatatcgatGTTCTTTACGATGCCATTGGGATAT pQE30 pBSV2G-flaBp2C→G-gfp
B172 tagcatgagcatgcCTGCAGGCGGCCGCACTAGTG gfp 3 ′ Multiple constructs
B197 ATGCGATCCTCTCATAGTTAATTTCTCCTC gfp DNA sequencing
B234 CGACAGGTAATGGTTGTCTG gfp pBSV2G-flaBp2C→G-gfp
a

gene sequences are in capitals and unrelated sequences are in small letters. Functional restriction enzyme sites added to the primers are underlined and mutations are underlined and bolded.