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. Author manuscript; available in PMC: 2009 Oct 19.
Published in final edited form as: Biochemistry. 2007 May 18;46(23):6822–6829. doi: 10.1021/bi700011d

Figure 8.

Figure 8

Identification of monoglutaminyl-BV as an intermediate of the pathway. (A) HPLC profile of midgut homogenates dissected 24 h after meal from females fed on plasma supplemented with 0.5 mM BV IXα. (B) ESI/MS analysis of AaBV produced upon feeding with BV IXα (indicated by an asterisk in the chromatogram). (C) ESI/MS analysis of a minor peak (arrow in Panel A) eluted between AaBV and BV IXα. (D) Fragmentation (tandem ESI-MS) spectrum of monoglutaminyl-BV IXα (m/z 711) produced a daughter-ion species at m/z 583, which, by further fragmentation (MS3) (E) gave origin to similar daughter-ion species as BV IXα (Figure 4B).