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NOS inhibition restores osteoclast differentiation in CD47−/− cells. A, WT and CD47−/− macrophages were differentiated into osteoclast in the presence of 50 ng/mL M-CSF and 50 ng/mL RANKL. RNA was isolated at days 1, 5, and 7, and cDNA was made. qPCR analysis was carried out with specific primers to iNOS. B, WT and CD47−/− macrophages were differentiated into osteoclasts in the presence of M-CSF, RANKL, and L-NAME, a pan-inhibitor of NOS, for 5 d.
