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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Cancer Res. 2009 Mar 10;69(7):3196–3204. doi: 10.1158/0008-5472.CAN-08-3358

Figure 5.

Figure 5

CD47−/− mice have decreased tumor burden and bone loss in an intracardiac metastasis model. A, B16-FL cells were injected into the left ventricular chamber of WT (n = 3) and CD47−/− (n = 3) mice. Tumor burden in the femur/tibia (P = 0.003) and the mandible (P = 0.05) as measured by bioluminescence imaging 7, 10, and 12 d after tumor cell injection. B, representative images of bioluminescence and of tibial histologic bone sections. M, marrow; T, tumor. C, tumor volume/total volume in the tibias of WT (n = 3) and CD47−/− (n = 3) mice at day 12 was measured by histomorphometric analysis (P = 0.02). Trabecular bone volume in the tibias of WT (n = 3) and CD47−/− (n = 3) mice at day 12 was measured by histomorphometric analysis. WT saline versus tumor, P < 0.01; CD47−/− saline versus tumor, P = 0.51. D, osteoclast perimeter in the tibias of WT (n = 3) and CD47−/− (n = 3) mice at day 12 was measured by histomorphometric analysis. Three independent experiments showed similar results.

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