Table 2.

Insulin tolerance test

Methods Checklist	Considerations in Experimental Design	Recommendations for Data Presentation
Body weight and body composition, if possible	Fast duration	Present entire time course of absolute glucose profile
Sex, age, and littermates if used	Group differences in body weight and body composition when insulin dose is calculated	Do not normalize glucose data on basis of differing basal glucose concentration
Insulin dose and type of insulin	Hematocrit changes if hand-held blood glucose meters are used	Present plasma insulin concentration
Route of insulin administration
Blood sampling site
Indicate if restraint or anesthesia was used
Diet and fasting status
Alterations in environment (temperature, light-dark cycle)

Considerations and recommendations are those specified by the authors.