Table 3.

Euglycemic hyperinsulinemic clamp

Methods Checklist	Considerations in Experimental Design	Recommendations for Data Presentation
Body composition and, if possible, body composition	Fast duration	Present entire time course of glucose concentrations and glucose infusion rates
Sex, age, and littermates, if used	Hematocrit changes if hand-held blood glucose meters are used	Present absolute rates of endogenous glucose production
Complete protocol for insulin and tracer infusion, including any priming boluses that are used	Group differences in body weight and body composition when insulin dose is calculated	Present actual basal and clamp plasma insulin concentrations
Flux calculations when appropriate	Predicted volumes of distribution and fractional turnover rates of insulin and glucose and predicted steady-state values when priming doses are calculated
Route of glucose, insulin and tracer administration
Blood sampling sites and frequency of collection
Indicate if restraint or anesthesia was used
Diet and fasting status
Alterations in environment (temperature, light-dark cycle)

Considerations and recommendations are those specified by the authors. Adapted from Wasserman et al. (27), with permission.