Fig. 3.

Recovery of phages displaying an MBP-binding monobody (filled bar) from selection with His-tagged MBP immobilized to magnetic beads through ^BTtris-NTA (the leftmost column). As a negative control, phages displaying a non-functional, ‘shaved’ FNfn10 in which residues in the BC and FG loops have been replaced with Ser (Koide et al., 2007) were used (open bar). The numbers of recovered phages are shown. The three right columns represent control experiments in which ^BTtris-NTA and/or the target were omitted.