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Effect of DTT on the immobilization of His-tagged targets tested via binding of monobody-displaying phages. His-tagged MBP (for the M4 monobody) and ySUMO (for the Y12 monobody) were immobilized to neutravidin-coated wells of a microtiter plate and binding of MBP- and ySUMO-binding monobodies displayed on phages were tested in the presence and absence of 1 mM DTT. Bound phages were detected with an anti-phage antibody. The identities of the two monobody clones are shown in Fig. 2.
