Fig. 3.

LC inhibits iNOS dimerization. A: Caco-2 monolayers were exposed to cytomix for the times indicated in the figure, and total protein extracts were prepared and analyzed by Western blotting. B: after fractionation of the nonreduced whole Caco-2 cell lysates by filtration with 200-kDa molecular weight cutoff (MWCO) ultrafilters, each compartment was reconstituted to the same volume and proteins were reduced by boiling with loading buffer containing 2-mercaptoethanol. Dimeric and monomeric iNOS was detected by Western blot analysis of the >200-kDa and <200-kDa compartments, respectively.