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. 2009 Oct;297(4):G840–G848. doi: 10.1152/ajpgi.90716.2008

Fig. 5.

Fig. 5.

The iNOS-suppressing factor in liver cytosol is arginase. Caco-2 monolayers were exposed to cytomix for 24 h, and supernatants were assayed for the release of NO2/NO3 by the Griess assay. A: addition of 5 mM Arg completely blocked the ability of LC to prevent NO production. B: 1 mM S-(2-boronoethyl)-l-cystein (BEC) completely blocked the ability of LC to prevent NO production. C: LC (400 μg/ml) was or was not sonicated before addition to supernatants of immunostimulated Caco-2 cells in the presence or absence of either mouse control IgG (50 μg/ml) or anti-arginase type I (Arg-1) antibodies (50 μg/ml). Antibodies were added at the start of the cytomix stimulation. Anti-Arg-1 antibody significantly decreased the ability of sonicated but not nonsonicated LC to decrease NO production from immunostimulated Caco-2 monolayers. D: urea concentration in the supernatants of immunostimulated Caco-2 cells was measured with or without LC (400 μg/ml). Addition of LC resulted in near maximal accumulation of urea within 9 h of culture stimulation. *P < 0.05 vs. control.