Table 4.
Tested compounds that do not elevate [Ca2+]i in P2Y5- and GαΔ6qi5myr-cotransfected CHO cells
| Name | Concentration |
|---|---|
| 18:1-PC | NE ≤100 μM |
| Tetradecyl phosphonate* | NE ≤10 μM |
| Oleic acid | NE ≤20 μM |
| Platelet activating factor* | NE ≤80 μM |
| ATP | NE ≤100 μM |
| dATP | NE ≤100 μM |
| Protein hydrolysate | NE ≤50 mg/ml |
| Farnesyl diphosphates* | NE ≤10 μM |
| Geranylgeranyl diphosphates | NE ≤10 μM |
| Geranyl diphosphates | NE ≤10 μM |
| Farnesol | NE ≤10 μM |
CHO cells were transfected with mtAEQ, P2Y5 cDNA (or the empty vector), and GαΔ6qi5myr. P2Y5-specific [Ca2+]i induction was determined as described in Table 3. PC, phosphatidylcholine. NE, not effective.
Tested for antagonism against lysophosphatidic acid (LPA).