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. 2009 Oct;297(4):G806–G813. doi: 10.1152/ajpgi.00279.2009

Fig. 5.

Fig. 5.

Depalmitoylation by 2-bromopalmitate (2-BP) dislocates palmitoyl-PSD95, PSD95, and nNOSα from varicosity membrane. A: 100 μM 2-BP causes depletion of palmitoyl-PSD95 from the varicosity membrane. B: depalmitoylation by 2-BP (100 μM) completely dissociates PSD95 from the membrane. C: 2-BP (100 μM) causes dislocation of 320-kDa nNOSα dimer from the membrane. The nNOS immunoblots were developed by cold SDS-PAGE. 2-BP treatment did not have any effect on nNOSα monomer and dimer in the varicosity cytosolic fractions. Untreated lysates or palmitate treatment served as controls.