Table 1.

PCR primers used

	Sequence	Product Size, bp	Accession Number
T1R1
sense	5′-TTGGTCTACTGCTGGGCTTT-3′	414	NM053305
antisense	5′-AGCGTGGTCAGTGTTGTCAG-3′
T1R2
sense	5′-AACAACACGGTCCCTGTCTC-3′	439	XM001074791
antisense	5′-GGCAGAAGCATGAGAAGACC-3′
T1R3
sense	5′-GAGGCCACTCTCAACCAGAG-3′	477	NM130818
antisense	5′-CCAGCTGAAATTCTGCAACA-3′
mGluR1
sense	5′-GCTCCTGGATTTCCTCATCA-3′	216	NM017011
antisense	5′-CGTACCATTCCGCTTTTGTT-3′
mGluR4
sense	5′-GATATTGTCCGAGCCCTCAA-3′	255	NM022666
antisense	5′-CTCCAACACCCTCCTGATGT-3′
CaSR
sense	5′-TCTTGTGGAGTGGGTTCTCC-3′	353	NM016996
antisense	5′-GGCCTTGACGATAGGTGTGT-3′
β-actin
sense	5′-GTGGGCCGCCCTAGGCACCA-3′	241	NM031144
antisense	5′-TGGCCTTAGGGTTCAGAGGG-3′

T1R, taste receptor type 1; mGluR, metabotropic glutamate receptor; CaSR, calcium-sensing receptor.