Fig. 2.
Impact of maturation and long-term hypoxia (LTH) on EFS-evoked [Ca2+]i transients in the absence and presence of the RyR antagonist ryanodine in isolated ovine SCG cells. Cells were activated by EFS as per protocol 1 in the absence and presence of 100 μM ryanodine (see Fig. 1A and materials and methods). A: normoxic fetal cells; B: normoxic adult cells; C: LTH fetal cells; D: LTH adult cells. Data are means ± SE; n = 12–19 cells from 6–8 animals/groups. *Significantly greater than ryanodine-treated cells (P < 0.05 by paired t-test).