Table 1.
Search for modulators of G-protein signaling in taste papillae.
| Prey | Bait | |||||
|---|---|---|---|---|---|---|
| Gαgus | Gαgus QL | Gαt2 | Gαi2 | Gαolf | Gs | |
| Ric-8A | ++ | +++ | ++++ | |||
| Ric-8B | + | ++++ | +++ | |||
| Ric-8BD9 | + | |||||
| AGS1 | ||||||
| AGS2 | ||||||
| AGS3 (GPR1-4) | + | ++++ | ||||
| AGS4 | + | |||||
| PBP | ||||||
| Pins/LGN (GoLoco) | +++ | + | ||||
| RGS2 | ||||||
| RGS9 (RGS) | ||||||
| RGS21 | + | |||||
Interaction between various modulators of G-protein signaling and G-protein alpha subunits was tested using the Proquest yeast two-hybrid system. Interaction was scored on minimal medium plates containing 25 mM 3-AT and lacking leucine histidine and tryptophan. Growth represented by stars in the table reflects the strength of the interaction. + weak, ++ moderate, +++ strong, ++++ very strong. Note that AGS3 and PBP interact strongly with Gαi2 as previously reported (Bernard et al., 2001) however except for Ric-8A none of the proteins tested interacts with gustducin under these conditions.