Skip to main content
. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Arch Biochem Biophys. 2009 Aug 23;490(2):145–157. doi: 10.1016/j.abb.2009.08.013

Fig. 1.

Fig. 1

Construction of the pGL2-TK and pE1A-pcDNA3 plasmid vectors. A: The pGL2-TK plasmid was made by cloning the HSV-1 TK promoter, isolated after BglII and HindIII double digestion of the pRL-TK plasmid, in front of the firefly luciferase ORF in the pGL2 Basic plasmid which lacks any promoter/enhancer element. B: The pE1AcDNA3 plasmid was generated by first amplifying by PCR the E1A region (consisting of part of the 5′ non-transcribed region and the whole coding region) from the genomic DNA of HEK293 cells using primers to which linkers containing restriction sites for NheI and BamHI were added, and then cloning the E1A amplicon into the pcDNA3.1.myc-His(−)A plasmid downstream of the CMV promoter.