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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Mol Immunol. 2009 Jun 21;46(13):2493–2504. doi: 10.1016/j.molimm.2009.05.022

Fig. 2.

Fig. 2

Signaling pathways activated following rHagB stimulation of DC. DC were stimulated with 40 µg/ml rHagB for 10, 30, 60 or 120 min. Following stimulation, cells were lysed and whole cell lysates were assessed for phosphorylation of (A) JNK, ERK1/2 and p38, (B) Akt and GSK3α/β and (C) NF-κBp65, IκBα and CREB by Western blot. Total p38 (A) and GSK3β (B) were used as loading controls. Unstimulated DC (far left lane) or DC stimulated with 100 ng/ml E. coli K12 LPS for 60 min (far right lane) were used as controls. Results represent one of three independent experiments.