Fig. 3.
Cytokine production by DC stimulated with rHagB is dependent on more than one signaling pathway, while GSK3 signaling regulates IL-10. DC (2×105) from WT mice were treated with 10 µM of U0126 (left striped bar), SB203580 (grey bar), NF-κB SN50 (black bar), or GSK-3 Inhibitor IX (right striped bar) (ERK1/2, p38, NF-κB, or GSK3 specific inhibitors, respectively) for 2 h. Untreated cells were used as a negative control (white bars). The cultures were then stimulated with 40 µg/ml rHagB, 100 ng/ml E. coli K12 LPS, or left unstimulated. Culture supernatants were harvested 24 h post-stimulation and assessed for the production of TNF-α, IL-6, IL-12p40 and IL-10 by ELISA. Results are expressed as the mean ± standard error of triplicate cultures from one of two independent experiments. *** Significant differences at P < 0.001, compared to untreated cultures stimulated with rHagB or LPS.