Cytokine production by rHagB activated DC is mediated through TLR4 signaling and requires both MyD88 and TRIF. DC (2×105) from WT, TLR2−/− and TLR4−/− mice (A, C) or WT, MyD88−/− and TRIF Lps2 mice (B, C) were stimulated with 10, 20 or 40 µg/ml rHagB for 24 h. Culture supernatants were then harvested and assessed for TNF-α, IL-6, IL-12p40 (A, B) and IL-10 production (C) by ELISA. Results are expressed as the mean ± standard error of triplicate cultures from one of four independent experiments. *** Significant differences at P < 0.001 compared to WT cultures stimulated with rHagB.