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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Exp Hematol. 2009 Aug 26;37(11):1340–1352.e3. doi: 10.1016/j.exphem.2009.08.004

Figure 2.

Figure 2

NIC increases Mk ploidy under diverse culture conditions. (A-C) The percentage of CD41+ cells with DNA content ≥ 8N (high-ploidy Mks) in cultures initiated with mPB CD34+ cells. (A) Cells were grown in fibronectin (FN)-coated or control well plates in media containing 100 ng/mL Tpo. On day 5, 6.25 mM NIC was added to a subset of the wells. Entire wells were sacrificed to determine Mk ploidy. (B) Cells were cultured with 100 ng/mL Tpo with or without 150 ng/mL SDF-1α beginning at day 0. Where indicated, 6.25 mM NIC was added at either day 2 (SDF-1α culture) or day 5. (C) Cells were cultured with 100 ng/mL Tpo with or without 100 ng/mL SCF beginning at day 0. At day 5, the indicated cultures were supplemented with 6.25 mM NIC. (D) mPB CD34+ cells were cultured with IL-3, IL-6, and SCF (Cocktail). Beginning on day 5, either 100 ng/mL Tpo, 6.25 mM NIC, or Tpo + NIC was added to the cultures. The histograms show the DNA content (stained with PI) of CD41+ cells at day 11. (A-D) The data shown are representative of two biological experiments.