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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Dev Biol. 2009 Jul 28;335(1):253–262. doi: 10.1016/j.ydbio.2009.07.033

Figure 5. Novel PAR-1 localization in species of the Protorhabditis group.

Figure 5

Fluorescent staining of embryos with PAR-1 antibodies (green) and DAPI (blue). (A) PAR-1 localizes exclusively to the posterior half of the cortex in wild type C. elegans one-cell stage embryos (Kemphues, 2000). (B) PAR-1 localizes all around the cortex in a one-celled C. elegans par-6(RNAi) embryo (Guo and Kemphues, 1996), which ultimately leads to the ectopic spindle rotation in AB and the four-cells-in-a-row phenotype. (C,D) PAR-1 localizes all around the cortex at the one-cell stage in Diploscapter sp. (JU359) and Protorhabditis sp. (JB122) embryos. (E, F) During the two-cell (E) and four-cell (F) stage in Protorhabditis sp. (JB122), PAR-1 becomes asymmetrically localized in the germline precursor P1 and P2, just like in C. elegans (Guo and Kemphues, 1996).