Table 2.
Categories of yeast deletion mutants sensitive to arsenite. The deletion mutants that showed arsenic-sensitive phenotype were categorized based on the biological functions using FunSpec. The category was ordered by p value. Some of these mutants are present in more than one category as they have several functions.
| Functional Category | p-value | In category from cluster | Arsenite Toxitciy Modulating |
Total in Category |
|---|---|---|---|---|
| vacuolar/lysosomal transport[20.09.13] | 3.31E-08 | VPS8 STP22 FEN1 CUP5 VPS25 VPS24 VPS51 SNF7 SRN2 PEP3 VPS36 VMA6 MVP1 VPS21 VMA4 VTS1 SNF8 VPS16 BRO1 |
19 | 153 |
| cytoskeleton/structural proteins[42.04] | 4.14E-07 | HSL7 BEM1 STE50 RES161 RPN4 RVS167 GIM4 PAC10 PFD1 SAC1 YKE2 TUB3 CLA4 SVL3 NIP100 |
15 | 113 |
| modification by acetylation, deacetylation [14.07.04] |
2.47E-06 | SGF29 MAK31 ADA2 SGF73 GCN5 ARD1 HDA1 PHO23 HFI1 MAK3 HDA3 |
11 | 69 |
| cell growth / morphogenesis [40.01] | 1.66E-05 | SLA1 HSL7 STE50 RVS161 FEN1 RPN4 HBT1 REG1 BMH2 SSD1 RVS167 HOC1 GRR1 CLA4 STI1 SVL3 NIP100 |
17 | 189 |
| regulation of C-compound and carbohydrate metabolism [01.05.25] |
4.11E-05 | REG1 PBS2 GRR1 VPS25 SNF7 HOG1 VPS36 PSY2 POP2 GAL11 SNF2 SNF8 SSN3 |
13 | 126 |
| protein binding [16.01] | 6.77E-05 | SLA1 PIN4 BEM1 STP22 RVS161 BMH2 MSN5 RVS167 GIM4 UBC8 PAC2 PAC10 RTT101 PBS2 PFD1 GRR1 VPS51 UBI4 SIC1 PFP3 YKE2 THP1 STI1 RBL2 NIP100 |
25 | 391 |
| endocytosis [20.09.18.09.01] | 0.0001976 | SLA1 EDE1 RVS161 DOA4 RVS167 CUP5 VPS21 SVL3 |
8 | 59 |
| osmosensing and response [34.11.03.13] | 0.0003469 | STE50 DOA4 PBS2 HOG1 NST1 SSK2 | 6 | 35 |
| M phase [10.03.01.01.11] | 0.0004621 | SPC72 DOC1 TUB3 CIK1 MCK1 CSE2 NIP100 | 7 | 51 |
| trapnsport ATPases [ 20.03.22] | 0.0005872 | PCA1 CUP5 SPF1 PMR1 PPA1 VMA6 VMA4 | 7 | 53 |
| protein targeting, sorting and translocation [14.04] |
0.000737 | VPS8 STP22 PEX19 MSN5 CUP5 KAP123 VPS25 VPS24 VPS51 SNF7 SRN2 PEP3 VPS36 MVP1 VPS21 VTS1 SNF8 VPS16 |
18 | 281 |
| purine nucleotide/nucleoside/nucleobase anabolism [01.03.01.03] |
0.00107 | ADE1 YSA1 ADE6 YNK1 BAS1 | 5 | 29 |
| vacuole or lysosome [42.25] | 0.001227 | VAC17 KCS1 DOA4 CUP5 PEP3 VPS16 | 6 | 44 |
| modification by ubiquitination deubiquitination [14.07.05] |
0.001451 | DOA4 UBC8 UBP3 RTT101 GRR1 UBI4 BRE5 UBP2 | 8 | 79 |
| homeostasis of protons [34.01.01.3] | 0.001743 | CUP5 PPA1 VPH2 MEH1 VMA6 VMA4 | 6 | 47 |
| proteasomal degradation (ubiquitin/proteasomal pathway) [14.13.01.01] |
0.0028 | RPN4 UBC8 BST1 DOC1 PRE9 RPL40A RTT101 GRR1 DOA1 BRO1 |
10 | 128 |
| vacuolar protein degradation [14.13.04.02] | 0.002932 | APE3 VID30 MEH1 | 3 | 11 |
| phosphate metabolism [01.04] | 0.00345 | YSA1 NPP1 RBK1 DUN1 KCS1 REG1 PRO1 DBF2 YTA4 KSP1 PBS2 YNK1 PEX1 SAC1 HOG1 PPZ1 CLA4 MCK1 PEX6 SSK2 SSN3 |
21 | 401 |
| stress response [32.01] | 0.005175 | NTH1 KCS1 SSD1 PBS2 GRR1 UBI4 TRM9 PPZ1 MCK1 SSK2 BRO1 |
11 | 162 |
| osmotic and salt stress response [32.01.03] |
0.005561 | STE50 REV161 RVS167 HOG1 NST1 GRX5 | 6 | 59 |
| budding, cell polarity and filament [43.01.03.05] |
0.005666 | SLA1 HSL7 BEM1 STE50 RVS161 FEN1 RPN4 BMH2 SSD1 RVS167 BEM2 HOC1 GRR1 CLA4 THR1 SVL3 NIP100 |
17 | 312 |
| cytoplasmic and nuclear protein degradation [14.13.01] |
0.006041 | DOA4 UBP3 YTA7 CPS1 UBI4 UBP2 | 6 | 60 |
| MAPKKK cascade [30.01.05.01.03] | 0.006082 | STE50 PBS2 HOG1 SSK2 | 4 | 27 |