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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Chem Res Toxicol. 2009 Oct;22(10):1680–1688. doi: 10.1021/tx900090m

Figure 3.

Figure 3

Reverse phase HPLC of trypsin-digested human butyrylcholinesterase labeled with thiomethyl sarin Rp. The 0.18 mg of labeled human butyrylcholinesterase (2.2 nanomoles) was digested with trypsin and loaded onto a 100 × 4.6 mm Phenomenex C18 column. Peptides were eluted with a 60 min gradient, from 0 to 60% acetonitrile, 0.1% trifluoroacetic acid at a flow rate of 1 mL/min. A 0.5 μl aliquot from each 1 mL fraction was examined in the MALDI-TOF mass spectrometer to identify the fraction that contained the active site peptide. The inset summarizes the elution times for unlabeled peptide and for peptides labeled with tabun, GF, sarin, or soman.