. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: Chem Res Toxicol. 2009 Oct;22(10):1728–1735. doi: 10.1021/tx900238t

Table 2.

Adduct levels in hepatic DNA of rats treated with NPYR^a

Dose (mg/kg bw)	7,8-Butanoguanine 6 (μmol/mol Gua)	Adduct 9			N²-(4-HOB)dGuo (18)^b (μmol/mol dGuo)	N⁶-(4-HOB)dAdo (19)^b (μmol/mol dAdo)	O²-(4-HOB)dThd (20)^b (μmol/mol dThd)
Dose (mg/kg bw)	7,8-Butanoguanine 6 (μmol/mol Gua)	(6S,8S)-(μmol/mol dGuo)	(6R,8R)-(μmol/mol dGuo)	Total (μmol/mol dGuo)	N²-(4-HOB)dGuo (18)^b (μmol/mol dGuo)	N⁶-(4-HOB)dAdo (19)^b (μmol/mol dAdo)	O²-(4-HOB)dThd (20)^b (μmol/mol dThd)
46	952 ± 180	0.154 ± 0.057	0.077 ± 0.021	0.231 ± 0.078	0.369 ± 0.134^c	0.013 ± 0.006	1.065 ± 0.349
92	1742 ± 245	0.341 ± 0.066	0.158 ± 0.026	0.499 ± 0.091	0.478 ± 0.114^c	0.008 ± 0.004	2.049 ± 0.361
184	3032 ± 855	0.634 ± 0.188	0.271 ± 0.054	0.905 ± 0.240	0.951 ± 0.193^c	0.016 ± 0.008	3.767 ± 0.840

Rats were given a single dose of NPYR by gavage and sacrificed 16 h later. Limits of quantitation were: 6, 0.1 μmol/mol Gua; 9, 0.004 μmol/mol dGuo; 18, 0.02 μmol/mol dGuo; 19, 0.002 μmol/mol dAdo; 20, 0.01 μmol/mol dThd.

DNA was treated with NaBH₃CN during enzyme hydrolysis to convert adducts 13–15 to 18–20.

Control amount was subtracted from each value.