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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Curr Opin Struct Biol. 2009 Sep 18;19(5):623–631. doi: 10.1016/j.sbi.2009.08.001

Figure 1.

Figure 1

Real-space variance of Thermus thermophilus 70S ribosome in which the ternary complex of elongation factor Tu (EF-Tu), tRNA and guanine nucleotide has been trapped on the ribosome using the antibiotic kirromycin [44]. The structure was originally determined to 6.4 Å from a data set of 323,688 2D projection images that were pre-selected by multi-reference refinement from a total of 586,329 projection images. The 3D variance map was calculated from 170,000 bootstrap volumes. The regions of high variance include location of E-site tRNA and L1 protuberance (left) and also part of the stalk base (right) showing there is still localized heterogeneity in these regions in agreement with our previous analysis [44].