Figure 1.

Photostability comparison between EGFP and mTFP1. EGFP (white circles) and mTFP1 (black squares) intensity profile measured in two monotransfected cells as a function of illumination time under the microscope are presented. The time delay between each acquisition was set to 10 s. The system was equipped with 440 nm ± 10 and 475 nm ± 20 band-pass filters, and we calculated an average power of 1.65 mW and 1.93 mW at the exit of the objective for mTFP1 and EGFP, respectively. We obtained similar half-life times of photobleaching for both proteins (in this case, we recovered t_1/2(mTFP1) = 132 s and t_1/2(EGFP) = 205 s).