Table 1.
Fraction of interacting donor (fD) and FRET efficiency (E) for tandems using a discrete double exponential model with fixed donor
| Tandem | fD | E |
|---|---|---|
| TdRed-EGFP | 0.77 ± 0.14 | 0.64 ± 0.01 |
| mRFP1-EGFP | 0.26 ± 0.08 | 0.56 ± 0.02 |
| HaloTag(TMR)-EGFP | 0.23 ± 0.09 | 0.56 ± 0.04 |
| HaloTag(TMR)-EGFP∗ | 0.39 ± 0.06 | 0.51 ± 0.05 |
| mStrawberry-EGFP | 0.37 ± 0.07 | 0.58 ± 0.02 |
| mCherry-EGFP | 0.45 ± 0.02 | 0.58 ± 0.03 |
| mTFP1-mOrange | 0.37 ± 0.01 | 0.68 ± 0.02 |
| mTFP1-EYFP | 0.71 ± 0.01 | 0.61 ± 0.08 |
Standard deviation was calculated for a population of five different cells for each tandem. HaloTag(TMR)-EGFP experiments were obtained with ligand (TMR) incubation of 15 min, whereas for HaloTag(TMR)-EGFP∗ the incubation time was 24 h.