Figure 3.

Concentration dependency of the size exclusion chromatographs of peptides. Peptides were eluted from the column in 200 mM sodium phosphate, pH 7.00, and at room temperature (∼22°C), and were loaded onto the Superdex Peptide column at various concentrations. (A) R₃GQ₃GY at 10 μM (orange), 60 μM (red), 100 μM (blue), 455 μM (green), 3.03 mM (black), and 5.88 mM (cyan). The inset shows a magnified view of the region of the chromatograph in which the oligomers elute. (B) R₃GQ₃P₁₁GY at 10 μM (orange), 60 μM (red), 100 μM (blue), 455 μM (blue), and 2.75 mM (black). (C) R₃GP₁₁GY at 10 μM (orange), 45 μM (red), 100 μM (blue), 455 μM (green) and 2.25 mM (black). (D) R₃AQ₃AY at 10 μM (orange), 37 μM (red), 100 μM (blue), 455 μM (green), and 1.87 mM (black). Flow rate was 0.5 mL/min, void volume was at 15.4 min, and total volume was at 35.9 min.