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SOSG fluorescence in optically trapped microspheres. (Top) Brightfield images. (Center) Fluorescence images at 535 nm. (Bottom) Fluorescence image line scans. (A) 0.79-μm SA polystyrene microsphere without SOSG. (B) No microsphere with SOSG. (C) 0.79-μm SA polystyrene microsphere with SOSG. (D) 2.1-μm SA polystyrene microsphere with SOSG. (E) 0.78-μm silica microsphere with SOSG. Scale bar = 1 μm. A trap power of 390 mW was used in all the images.
