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. 2009 Oct 28;4(10):e7640. doi: 10.1371/journal.pone.0007640

Figure 5. Human hSPC105 is essential for chromosome segregation, mitotic timing, cytokinesis, and spindle checkpoint activity.

Figure 5

(A) Immunofluorescence imaging of mitotic HeLa cells following treatment with control or targeting siRNA oligos. Cells were stained with anti-hSPC105 antibodies (green), CREST sera (red) and DAPI (DNA, blue). Scale Bar: 10 µm. Insets are 13× magnified. (B) DAPI stained mitotic HeLa cells treated with hSPC105 targeting or control siRNA oligos. (C) Time-lapse imaging of histone 2B-GFP-expressing HeLa cells treated with hSPC105 targeting or control siRNA oligos. Images were collected every 3 min from ∼35 cells; representative frames are shown. In control cells (upper lane) anaphase occurs only after sister chromatid congression is complete, while in hSPC105-depleted cells (lower lane) anaphase is initiated in the presence of unaligned sisters, generating chromosome bridges (yellow arrow). (D) Cell division defects quantified from live-cell imaging experiments. (E) Percentage of nocodazole-treated control and hSPC105-depleted HeLa cells arresting in mitosis (as judged morphologically by DIC microscopy).